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488 nm: A Review of FACS Technology and its Application in Biological Research

488 nm: A Review of FACS Technology and its Application in Biological Research

Fluorescent imaging depends on the physical principles behind the scattering of light. In a typical fluorescent microscope, a laser, usually of argon, shines through a sample at a wavelength of 488 nm. The sample, in turn, fluoresces according to how it was stained. Certain cells, and often even specific cellular structures, can be resolved, which results in a fluorescent image that is reflected into an optical lens or screen for visual analysis.

A Microscopic Arms Race: The Battle Against Antibiotic Resistance

The overuse of antibiotics has led to the evolution of resistant strains of bacteria. Strains now exist that elude even the strongest antibacterial agents. The World Health Organization estimates that two million people in the United States are infected with antibiotic- resistant bacteria, resulting in the deaths of 14,000 each year. Once an indispensable weapon in the fight against disease, antibiotics have ushered in a new public health threat.

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